Nom du corpus

Corpus Systématique Animale

Titre du document

DNA methylation of sex chromosomes in a dioecious plant, Melandrium album

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • DNA methylation
  • In situ nick-translation
  • Lyon hypothesis
  • Sex chromosomes
  • Melandrium album
Nom du fichier dans la ressource
Mammiferes_v2b_00460
Auteur(s)
  • Boris Vyskot 1
  • Alejandro Araya 2
  • Jacky Veuskens 3
  • Ioan Negrutiu 1
  • Armand Mouras 1,2
Affiliation(s)
  • 1) Laboratoire de Biologie Cellulaire, Université de Bordeaux II, F-33405, Talence Cedex, France
  • 2) Institut de Biochimie Cellulaire, CNRS, F-33077, Bordeaux Cedex, France
  • 3) Plamengenetica, Instituut voor Moleculaire Biologie, B-1640, St Genesius Rode, Belgium
Résumé

Melandrium album, a dioecious plant species, has two heteromorphic sex chromosomes with the XY constitution typical for male and the XX for female plants. This plant represents an experimental model system of sex determination in which the Y chromosome plays a strongly dominant male role. We present data on the overall transcriptional activities of M. album sex chromosomes. DNA methylation patterns were analysed directly at the level of chromosomes using in situ nick-translation of fixed root mitotic chromosomes after nuclease digestion and in vivo labelling with S-adenosyl-l-[methyl-3H] methionine as donor of methyl groups. Both techniques revealed that the two X chromosomes of female plants had different levels of DNA methylation. Cell treatment with a DNA hypomethylating drug, 5-azacytidine, significantly influenced the labelling densities. These results imply that in female M. album plants, one of the two X chromosomes may be hypermethylated and inactive as described for mammalian cells (Lyon hypothesis). A similar analysis made on male cells displayed a similar relative levels of methylation in autosomes and sex chromosomes, thus indicating the transcriptional activity of both Y and X male chromosomes.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - genetics & heredity
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - cytologie, morphologie, systematique, floristique et evolution des vegetaux
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
Identifiant ISTEX
8636B66F0E6B9428744579798A601F568861976D
Revue

Molecular and General Genetics MGG

Année de publication
1993
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.967
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-KG5RXJLG-S
Powered by Lodex 9.3.8