Nom du corpus

Corpus Systématique Animale

Titre du document

Properties of an acid cholesteryl ester hydrolase inhibitor from rat serum

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_01531
Auteur(s)
  • Mitsuo Tanaka 1
  • Toshihiro Iio 1
  • Toshikazu Tabata 1
Affiliation(s)
  • 1) Showa College of Pharmaceutical Sciences, 3-3165, Higashi-tamagawagakuen, 194, Machida-City, Tokyo, Japan
Résumé

The inhibitory effect of a protein isolated from rat serum on lysosomal acid cholesteryl ester hydrolase (acid CEH; EC.3.1.1.13) activity was studied. An inhibitor was purified from rat serum following ultracentrifugation and heat treatment using column chromatography on Sephacryl S-200 and ultrafiltration. The purified inhibitor appeared as a single protein band in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The molecular weight of the inhibitor was 28,000 Daltons as judged by gel filtration on Sephacryl S-200 and SDS-polyacrylamide gel electrophoresis. The purified inhibitor was shown to be apolipoprotein A-I (apo A-I), the major apolipoprotein of high-density lipoprotein (HDL), using immunoprecipitation with rat anti-apo A-I immunoglobulin (Ig)G. Inhibition of acid CEH activity by apo A-I was dependent on the concentration of apo A-I. The values of Vmax obtained were similar with or without apo A-I. Apo A-I of various other mammalian species, including human, bovine and rabbit, also inhibited acid CEH activity. Other apolipoproteins, such as apo A-II and apo B, also showed inhibiting activity. On the other hand, apo A-I had no effect on the activity of other enzymes found in lysosomes, such as cathepsin D, ?-glucuronidase and acid phosphatase. The results suggest that apolipoproteins may play a role in the regulation of hydrolysis of cholesteryl esters in lipoproteins, that have been transferred to the liver, and that the inhibition of acid CEH activity by apo A-I may be a characteristic of the lipid-binding protein or be due to changes of the lipid/water interface.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - nutrition & dietetics
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Physical Sciences ; 2 - Chemistry ; 3 - Organic Chemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - nutrition & dietetics
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
134DF5E499C298274E6457AA325F5E89198B23A9
Revue

Lipids

Année de publication
1990
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.485
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-KB6W1TNS-8
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