Nom du corpus

Corpus Systématique Animale

Titre du document

Biochemical and cytological studies of bleomycin actions on chromatin and chromosomes

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_01784
PMID
Auteur(s)
  • M. Tien Kuo 1
  • T. C. Hsu 1
Affiliation(s)
  • 1) Section of Cell Biology, The University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, 77030, Houston, Texas, USA
Résumé

Interaction of bleomycin with nuclei isolated from a variety of mammalian cells resulted in the release of nucleosomes. When isolated mononucleosomes (core plus linker) were re-treated with bleomycin, no further degradation of DNA occurred. The results suggest that the bleomycin cleavage sites in chromatin are present only in the linker region and that there are probably only one or two cleavage sites per linker. The repeat lengths of nucleosomal DNA released by bleomycin from nuclei of different species are different; this variability is considered to reflect the length of the linker. Incorporation of BrdU into DNA did not alter the bleomycin action on nucleosomes. When mitotic cells were held at metaphase for a prolonged period, bleomycin caused a gradual disintegration of chromosomes, although the bleomycin cleavage sites in metaphase chromosomes were found to be the same as those in interphase nuclei.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - developmental biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Genetics(clinical)
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
CD0EC3027343A3D1E4F45ADF810FF20402C49A29
Revue

Chromosoma

Année de publication
1978
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.49
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-JTD0KDD6-M
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