Nom du corpus

Corpus Systématique Animale

Titre du document

Polyphosphoinositides and the shape of mammalian erythrocytes

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00628
Auteur(s)
  • Eugene Quist 1
  • Patricia Powell 1
Affiliation(s)
  • 1) Department of Pharmacology, Texas College of Osteopathic Medicine, 76107, Fort Worth, TX
Résumé

The relationship between polyphosphoinositide and phosphatidic acid (PA) metabolism and Mg-ATP dependent shape and viscosity changes in erythrocyte ghosts from four mammalian species was examined. Ghosts prepared from rabbit, dog, human and guinea pig erythrocytes were transformed from echinocytes to discocytes within 15 min in the presence of 1 mM Mg-ATP at 25 C. In all species these Mg-ATP shape transformations were associated with a 30–45% decrease in the specific viscosity of the ghost suspensions. Mg-ATP induced a second transformation of discocytic ghosts to cup shape forms without a further decrease in viscosity. A considerable species variation in the rates of Mg-ATP dependent viscosity and shape changes and incorporation of32P into phosphatidylinositol-4? phosphate (PIP), phosphatidylinositol-4?5?bisphosphate (PIP2) and especially PA form MG-[? 32P]-ATP in ghosts was found. However, the rates of Mg-ATP dependent synthesis of PIP and PIP2 and shape and viscosity changes in each species were of the same magnitude. Ca2+ or neomycin strongly inhibited PIP labeling and Mg-ATP shape and viscosity changes in ghosts of the different species. Ca2+ or neomycin usually increased or had little effect on32P incorporation into PA and PIP2. The possibility that Mg-ATP-induced changes in erythrocyte membrane shape and deformability are dependent on increases in membrane PIP and PIP2 is discussed.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - nutrition & dietetics
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Physical Sciences ; 2 - Chemistry ; 3 - Organic Chemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - nutrition & dietetics
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
E1CE379985391738AF1AB5FE3CEFB46D81FDACC3
Revue

Lipids

Année de publication
1985
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.599
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-J665JWW0-Z
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