Nom du corpus

Corpus Systématique Animale

Titre du document

Growth of fish cell lines in glutamine-free media

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Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Fish cells
  • glutamine-free growth
  • nutrition
  • BSA : bovine serum albumin
  • CHSE-214 : Chinook samon embryo cell line
  • dFBS : dialyzed fetal bovine serum
  • FBS : fetal bovine serum
  • GFSk-S1 : goldfish skin cell line
  • GS : glutamine synthetase
  • L-15 : Leibovitz's L-15 media
  • L929 : mouse fibroblast cell line
  • MEM : minimum essential medium Eagle
  • PBS : phosphate buffered saline
  • RTL-W1 : rainbow trout liver cell line
  • RTSp-W1 : rainbow trout spleen cell line
Nom du fichier dans la ressource
Poissons_v2b_004967
Auteur(s)
  • N. C. Bols 1
  • R. C. Ganassin 1
  • D. J. Tom 1
  • L. E. J. Lee 2
Affiliation(s)
  • 1) Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada
  • 2) Department of Veterinary Anatomy, Western College of Veterinary Medicine, University of Saskatchewan, S7N 0W0, Saskatoon, SK, Canada
Résumé

The glutamine requirement for thein vitro proliferation of fish cells was investigated with cell lines from four different species and three tissues: goldfish skin (GFSk-S1), Chinook salmon embryo (CHSE-214), and raibow trout liver (RTL-W1) and spleen (RTSp-W1). With a supplement of fetal bovine serum, the basal medium, Leibovitz's L-15, without glutamine supported the proliferation of all four cell lines as well, or nearly as well, as L-15 with 2 mM glutamine. This was true over short term assays of two to four weeks and for continuous propagation. CHSE-214 also grew as well with or without 2 mM glutamine in Minimum Essential Medium with fetal bovine serum. However, when the supplement was dialyzed fetal bovine serum, CHSE-214 grew much better in L-15 without glutamine. Therefore, glutamine was not required for growth in L-15, and in fact, was inhibitory in the absence of the dialyzable fraction of serum. By contrast, glutamine appeared to be important for growth in Minimum Essential Medium. When the supplement was dialyzed fetal bovine serum, CHSE-214 grew much better in Minimum Essential Medium with 2 mM glutamine. These results suggest that the glutamine requirement for thein vitro proliferation of fish cells is conditional and depends on the basal medium and serum supplement.

Catégories Science-Metrix
  • 1 - applied sciences
  • 2 - enabling & strategic technologies
  • 3 - biotechnology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Physical Sciences ; 2 - Engineering ; 3 - Biomedical Engineering
  • 1 - Physical Sciences ; 2 - Chemical Engineering ; 3 - Bioengineering
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biotechnology
Catégories WoS
  • 1 - science ; 2 - cell biology
  • 1 - science ; 2 - biotechnology & applied microbiology
Identifiant ISTEX
7F2333668BD8AF667352BEB8349155DA7155F75A
Revue

Cytotechnology

Année de publication
1994
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.704
Sous-corpus
  • Poissons
Type de publication
Journal
ark:/67375/1BB-J4850H58-R
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