Nom du corpus

Corpus Systématique Animale

Titre du document

A new method for immunologically marking prey and its use in predation studies

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Predator
  • prey
  • ELISA
  • gut content analysis
  • methods
Nom du fichier dans la ressource
Insectes_v2b_01791
Auteur(s)
  • J. R. Hagler 1
  • C. M. Durand 1
Affiliation(s)
  • 1) Western Cotton Research Laboratory, USDA-ARS, 4135 East Broadway Road, 85040, Phoenix, Arizona, USA
Résumé

We introduce a new method for immunologically examining predator gut contents. It differs from previously described gut content analyses because it does not require the development of prey-specific antibody probes. Instead, insect prey were marked with a readily available antigen, rabbit immunoglobulin G (IgG). We then assayed predators that had fed on IgG labeled prey with an enzyme-linked immunosorbent assay (ELISA) using goat anti-rabbit IgG. Of the predator species that fed on the IgG labeled prey, 98.8% of those with chewing mouthparts scored positive for IgG 1 h after feeding. Our prey-labeling ELISA was less efficient for detecting IgG prey remains in predators with piercing/sucking mouthparts. Only 29.5% of these individuals scored positive for rabbit IgG in their guts 1 h after feeding. An additional study was conducted to measure the retention time of IgG-labeled prey in the guts of two species of predators with chewing mouthparts. Results from this experiment showed that the retention time varied depending on the predator and prey species examined. Results from these studies indicate that this marking technique could have widespread use for analyzing the gut contents of predators with chewing mouthparts, but it has limited application for those predators with piercing/sucking mouthparts.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - entomology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - General Agricultural and Biological Sciences
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Ecology, Evolution, Behavior and Systematics
Identifiant ISTEX
5B95E16D0CD09324D558D4CD2D59FF7CC1FBDE9D
Revue

Entomophaga

Année de publication
1994
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.969
Sous-corpus
  • Insectes
Type de publication
Journal
ark:/67375/1BB-HTDNFTKK-1
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