Nom du corpus

Corpus Systématique Animale

Titre du document

Purification of cadmium-binding proteins from related species of terrestrial helicidae (gastropoda, mollusca): A comparative study

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • cadmium
  • cadmium-binding protein
  • land snails
  • Helix pomatia
  • Cepaea hortensis
  • Arianta arbustorum
Nom du fichier dans la ressource
Mammiferes_v2b_00682, Mollusques_v2b_00253
Auteur(s)
  • Reinhard Dallinger 1
  • Burkhard Berger 1
  • Andrea Bauer-Hilty 1
Affiliation(s)
  • 1) Institut für Zoologie, Abteilung Zoophysiologie, Univ. Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria
Résumé

Three species of terrestrial Helicidae (Helix pomatia, Cepaea hortensis andArianta arbustorum) were fed cadmium-rich diet in the laboratory. The snails accumulated high amounts of the metal in their hepatopancreas. Most cadmium and some zinc were found, after centrifugation, in the soluble fractions from which a cadmium-binding protein was isolated for each species by ion exchange and gel chromatography. The proteins contained different amounts of cadmium, but little or no zinc, and showed high absorption at 254 nm indicating the presence of cadmium-mercaptide bonds. After gel filtration, a molecular weight of 12000 was found for cadmium-binding proteins fromHelix pomatia andArianta arbustorum, whereas a molecular weight of 10 000 was found for a cadmium-binding protein fromCepaea hortensis. SDS-polyacrylamide gel electrophoresis showed one single band for each protein fromHelix pomatia andArianta arbustorum and suggested a molecular weight of 11000 for both species. Amino acid analysis revealed, for each protein, high amounts of cysteine (12–20%), glycine (15–19%), and serine (12–14%), and moderately elevated contents of lysine (9–13%) and alanine (4–8%), but no methionine and only traces, if any, of aromatic amino acids. The ratios of cadmium to cysteine were 1:5, 1:10 and 1:3 in the proteins fromHelix pomatia,Cepaea hortensis andArianta arbustorum, respectively. Some features of the isolated proteins resembled mammalian metallothioneins. Most characteristics, however, differed from true metallothioneins and were similar to cadmium-binding proteins found in some marine molluscs.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
7ED6C569E5ADD47CB24F6DFA8B86FF231EC849C5
Revue

Molecular and Cellular Biochemistry

Année de publication
1989
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.632
Sous-corpus
  • Mammiferes
  • Mollusques
Type de publication
Journal
ark:/67375/1BB-HCG86P74-G
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