Nom du corpus

Corpus Systématique Animale

Titre du document

Immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (P4-5C)

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Immunohistochemistry
  • Salivary gland
  • Specific monoclonal antibody
  • ABO(H) blood group antigens
  • Species identification
  • Immunhistochemie
  • Speicheldrüse
  • spezifischer monoklonaler Antikörper
  • ABH-Blutgruppenantigene
  • Speziesidentifikation
Nom du fichier dans la ressource
Mammiferes_v2b_00023
Auteur(s)
  • T. Ohshima 1
  • T. Nagano 1
  • A. Kimura 2
  • F. Matsumura 3
  • K. Sodesaki 3
  • T. Tsuji 2
  • H. Maeda 1
Affiliation(s)
  • 1) Department of Legal Medicine, Kanazawa University School of Medicine, 13-1 Takara-machi, 920, Kanazawa, Japan
  • 2) Department of Legal Medicine, Wakayama Medical College, 27 Kyuban-cho, 640, Wakayama, Japan
  • 3) Department of Forensic Science, Saga Medical School, Nabeshima-machi, 840-01, Saga, Japan
Résumé

A novel mouse monoclonal antibody (P4-5C) has been developed which recognizes the core portion of the protein carrying ABO(H) blood group antigens in human saliva. This proved to be specific for human saliva using immunochemical investigations such as enzymelinked immunosorbent assay, Ouchterlony method and counter-immunoelectrophoresis. By licht and electron microscope studies with immunohistochemical techniques using this human saliva-Specific P4-5C as primary antibody, it was shown that P4-5C reacted specifically and exclusively with mucus from the mucous gland cells of human salivary glands. P4-5C reacted neither with the mucous gland cells of other primates (hamadryas baboon, Japanese monkey and Rhesus monkey) and four mammals (dog, cat, rabbit and mouse) nor with other human tissues. The epitope on the core portion of the ABO(H)-carrying protein was defined by P4-5C and could be discriminated from the epitope of ABO(H) blood group antigens using immunoelectronmicroscopy, although these 2 epitopes were localized relatively close to each other. The P4-5C monoclonal antibody can be also used for morphological species identification of tissue specimens from submandibular glands.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - clinical medicine
  • 3 - legal & forensic medicine
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Pathology and Forensic Medicine
Catégories WoS
  • 1 - science ; 2 - medicine, legal
Identifiant ISTEX
1CAC4BD9AF2406E622827EFB531E8E8A2A837A87
Revue

International Journal of Legal Medicine

Année de publication
1991
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.955
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-GWV1V1T8-K
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