Nom du corpus

Corpus Systématique Animale

Titre du document

Molecular characterization of a centromeric satellite DNA in the hemiclonal hybrid frog Rana esculenta and its parental species

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Springer (journals)
Langue(s) du document
Type de document
Mots-clés d'auteur
  • centromere
  • clonal reproduction
  • germ line
  • Rana esculenta complex
  • satellite DNA
Nom du fichier dans la ressource
  • Matilde Ragghianti 1
  • Francesca Guerrini 1
  • Stefania Bucci 1
  • Giorgio Mancino 1
  • Hansjürg Hotz 2
  • Thomas Uzzell 3
  • Gaston-Denis Guex 2
  • 1) the Dipartimento di Fisiologia e Biochimica, Laboratorio di Biologia cellulare e dello sviluppo, Via Carducci 13, I-56010, Ghezzano (Pisa), Italy
  • 2) the Zoologisches Museum, Universität Zürich-Irchel, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland
  • 3) the Department of Ecology, Ethology and Evolution, University of Illinois, 515 Morrill Hall, 505 South Goodwin Avenue, 61801, Urbana, IL, USA

Hybrid water frogsRana esculenta reproduce by hybridogenesis: one parental genome (ofRana lessonae) is excluded in the germ line, the other (ofRana ridibunda) is clonally transmitted to haploid gametes. The two parental species differ in that the amount of centromeric heterochromatin revealed by differential staining is much higher inRana ridibunda. An abundant, tandemly arrayed, centromeric satellite DNA, designated RrS1, is revealed inRana ridibunda genomes by the restriction endonucleaseStul, which generates a major repetitive sequence fragment of 300 and a minor one of 200 bp. This AT-rich (68%) satellite family is located at the centromeres of the five largest chromosomes (1–5) and of a medium to small heterobrachial one (8 or 9); it thus constitutes only part of the centromeric heterochromatin that characterizes allRana ridibunda chromosomes. RrS1 represents about 2.5% of the genome ofRana ridibunda; it may represent as little as 0.2% of the genome ofRana lessonae, and cannot be detected inXenopus laevis frogs orSalamandra salamandra andTriturus carnifex salamanders. Segments of the satellite sequence are similar to sequences of yeast centromeric DNA element CDEIII and of the mammalian CENP-B box. A role for RrS1 and other centromeric satellite DNAs in the germ line genome exclusion of the hybridogenetic frog hybrids, although suggested, has not yet been demonstrated.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - developmental biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - vertebres: endocrinologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX

Chromosome Research

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  • Mammiferes
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