Nom du corpus

Corpus Systématique Animale

Titre du document

Interspecific “common” repetitive DNA sequences in salamanders of the genus Plethodon

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Batraciens_v2b_00202
Auteur(s)
  • Shigeki Mizuno 1
  • Christine Andrews 1
  • Herbert C. Macgregor 1
Affiliation(s)
  • 1) Department of Zoology, School of Biological Sciences, University of Leicester, LE1 7RH, Leicester, England
Résumé

Intermediate repetitive sequences of Plethodon cinereus which comprised about 30% of the genomic DNA were isolated and iodinated with 125I. About 5% of the 125I-repetitive fraction hybridized with a large excess of DNA from P. dunni at Cot 20. About half of the 125I-DNA in the hybrids was resistant to extensive digestion with S-1 nuclease. The average molecular size of the S-1 nuclease-resistant fraction was about 100 nucleotide pairs. The melting temperature of the S-1 nuclease-resistant fraction was about 2° lower than that of the corresponding fraction made with P. cinereus DNA. These results are taken to indicate the presence in the genomes of P. cinereus and P. dunni of evolutionarily stable “common” repetitive sequences. The average frequency of repetition of the common repetitive sequences is about 6,000 × in both species. The common repetitive fraction is also present in the genomes of other species of Plethodon, although the general populations of intermediate repetitive sequences are markedly different from one species to another. The cinereus-dunni common repetitive sequences could not be detected in plethodontids belonging to different tribes, nor in more distantly related amphibians. The profiles of binding of the common repetitive sequences to CsCl or Cs2SO4-Ag+ density gradient fractions of P. dunni DNA suggested that these sequences consisted of heterogeneous components with respect to base compositions, and that they did not include large amounts of the genes for ribosomal RNA, 5S RNA, 4S RNA, or histone messenger RNA. — In situ hybridization of the 3H-labelled intermediate repetitive sequences of P. cinereus to male meiotic chromosomes of the same species gave autoradiographs after an exposure of seven days showing all 14 chromosomes labelled. The pattern of labelling appeared not to be random, but was impossible to analyse on account of the irregular shapes and different degrees of stretching of diplotene and prometaphase chromosomes. In situ hybridization of the same sequences to meiotic chromosomes from P. dunni gave autoradiographs after 60 d exposure in which all chromosomes were labelled. These heterologous in situ hybrids can only have involved the “common” repetitive sequences.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - developmental biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - ethologie animale
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Genetics(clinical)
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
FE0ABB8338596CDB6835C2D9F0D747C7EB645C11
Revue

Chromosoma

Année de publication
1976
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
10
Sous-corpus
  • Batraciens
Type de publication
Journal
ark:/67375/1BB-FT73MMML-4
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