Nom du corpus

Corpus Systématique Animale

Titre du document

Sarcoplasmic reticulum calsequestrins: Structural and functional properties

Lien vers le document
Springer (journals)
Langue(s) du document
Type de document
Mots-clés d'auteur
  • sarcoplasmic reticulum
  • skeletal muscle
  • cardiac muscle
  • calcium binding protein
  • calsequestrin
Nom du fichier dans la ressource
Batraciens_v2b_00127, Mammiferes_v2b_00689, Oiseaux_v2b_00740
  • Kenichi Yano 1,2
  • Angel Zarain-Herzberg 1,2
  • 1) Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Taché Avenue, R2H 2A6, Winnipeg, Manitoba, Canada
  • 2) Department of Physiology, University of Manitoba, 351 Taché Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

Calsequestrin is the major Ca2+-binding protein localized in the terminal cisternae of the sarcoplasmic reticulum (SR) of skeletal and cardiac muscle cells. Calsequestrin has been purified and cloned from both skeletal and cardiac muscle in mammalian, amphibian, and avian species. Two different calsequestrin gene products namely cardiac and fast have been identified. Fast and cardiac calsequestrin isoforms have a highly acidic amino acid composition. The amino acid composition of the cardiac form is very similar to the skeletal form except for the carboxyl terminal region of the protein which possess variable length of acidic residues and two phosphorylation sites. Circular dichroism and NMR studies have shown that calsequestrin increases its ?-helical content and the intrinsic fluorescence upon binding of Ca2+. Calsequestrin binds Ca2+ with high-capacity and with moderate affinity and it functions as a Ca2+ storage protein in the lumen of the SR. Calsequestrin has been found to be associated with the Ca2+ release channel protein complex of the SR through protein-protein interactions. The human and rabbit fast calsequestrin genes have been cloned. The fast gene is skeletal muscle specific and transcribed at different rates in fast and slow skeletal muscle but not in cardiac muscle. We have recently cloned the rabbit cardiac calsequestrin gene. Heart expresses exclusively the cardiac calsquestrin gene. This gene is also expressed in slow skeletal muscle. No change in calsequestrin mRNA expression has been detected in animal models of cardiac hypertrophy and in failing human heart.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - biologie moleculaire et cellulaire
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX

Molecular and Cellular Biochemistry

Année de publication
Présence de XML structuré
Version PDF
Score qualité du texte
  • Batraciens
  • Mammiferes
  • Oiseaux
Type de publication
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