Nom du corpus

Corpus Systématique Animale

Titre du document

Growth in vitro of cells from renal glomerulus difficulties in establishing long-term cultures of rat glomerular endothelium

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00916
Auteur(s)
  • Z. Hruby 1
  • R. P. Lowry 2
Affiliation(s)
  • 1) Department of Nephrology, Academy of Medicine, Wroc?aw, Poland
  • 2) Royal Victorial Hospital, McGill University, Montreal, Canada
Résumé

Rat glomeruli were isolated utilizing differential sieving technique. Establishment and maintenance of epithelial and mesangial cell lines originating from glomeruli were based upon their individual growth requirements and simple cloning procedures. Despite repeated passages cells retained their specific morphologic features in cultures for approximately six months. Our attempts to grow rat glomerular endothelial cells with use of the platelet-derived growth factor (PDGF), endothelial mitogen (EM), thrombin and fibroblast-conditioned medium ended in failure. Although occasionally endothelial-like cells were seen in glomerular outgrowths, we did not succeed in cloning and propagating them in subsequent passages. It is concluded that growthin vitro of each type of rat kidney glomerular cells is dependent on specific conditions and stimulants. Moreover, endothelial cells of rat renal tufts proved refractory to cultivation using methods reported effective with regard to other mammalian species.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - clinical medicine
  • 3 - urology & nephrology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Urology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Nephrology
Catégories WoS
  • 1 - science ; 2 - urology & nephrology
Identifiant ISTEX
83D4723C1987E06EBDD9D043B6F1385504075901
Revue

International Urology and Nephrology

Année de publication
1989
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.678
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-FM3WWV5M-J
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