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Corpus Systématique Animale

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Identification and isolation of the primary aggregation factor from the cell membrane of the sponge Geodia cydonium

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Springer (journals)
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Mots-clés d'auteur
  • aggregation factor
  • cell membrane
  • glycoproteins
  • sponges
Nom du fichier dans la ressource
  • Prof. Dr. Werner E. G. Müller 1
  • Jürgen Conrad
  • Rudolf K. Zahn
  • Monika Gramzow
  • Branko Kurelec 2
  • Gerhard Uhlenbruck 3
  • 1) Institut für Physiologische Chemie der Universität Mainz, Duesbergweg, D-6500, Mainz, West Germany
  • 2) Laboratory for Marine Molecular Biology, Institute Ruder Boskovic, Center for Marine Research, Rovinj, Yugoslavia
  • 3) Abt. Experimentelle Innere Medizin, Medizinische Universitätsklinik, 5000, Köln, West Germany

The primary aggregation factor (pAF) of sponge cells is a glycoprotein that is firmly associated with the cell membrane. Polyspecific antibodies (anti-GM) prepared from sera raised against membranes of cells from the siliceous sponge Geodia cydonium were found to inhibit initial aggregation of homologous cells. The inhibition of aggregation, caused by anti-GM was neutralized by pAF. The pAF had been successfully solubilized and enriched by affinity chromatography, gel filtration and density gradient centrifugation, if checked by polyacrylamide gel electrophoresis in the presence of urea. The Mr of the native pAF was approximately 40 000 as estimated by gel filtration; under denaturing conditions three protein species (Mr: 16 500, 15 500 and 13 500) were identified in the pAF preparation. The pAF was precipitable by Ca++ and did not cross-react with antisera against homologous purified secondary aggregation factor and lectin. It is mainly composed of protein (48.0%) and carbohydrate (50.2%). The isolated pAF restored the aggregation potency not only of factor-depleted Geodia cells but also of cells from other Demospongiae. However, the pAF displayed no aggregation enhancing effect on urea-treated cells from species belonging to the Calcispongiae or Hexactinellida. We hypothesize that in contrast to the secondary aggregation, the initial aggregation of Geodia cells is mediated by the one-component system, the bivalent and bifunctional pAF.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX

Molecular and Cellular Biochemistry

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