Nom du corpus

Corpus Systématique Animale

Titre du document

Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: use in various animals indicates functional role of myohemoproteins

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • myoglobin
  • hemoglobin
  • muscle
  • heart
  • microassay
  • mammals
  • birds
  • fish
Nom du fichier dans la ressource
Mammiferes_v2b_02070, Oiseaux_v2b_02246, Poissons_v2b_004022
Auteur(s)
  • Peter James O'Brien 1
  • Hua Shen 1
  • Laura Jill McCutcheon 1
  • Michael O'Grady 2
  • Philip J. Byrne 1
  • Hugh W. Ferguson 1
  • Mehdi S. Mirsalimi 1
  • Richard J. Julian 1
  • Janice M. Sargeant 1
  • Robert R. M. Tremblay 2
  • Tim E. Blackwell 2
Affiliation(s)
  • 1) Departments of Pathology and Population Medicine
  • Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
  • 2) Departments of Pathology, Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario
Résumé

A novel, simple, rapid, sensitive and reproducible microassay is described for determination of myoglobin and hemoglobin content of myocardial and skeletal muscle biopsy specimens from various mammals, birds and fish. As little as 50 mg of tissue is needed and myoglobin concentrations lower than 1 mg% can be detected. Myoglobin and hemoglobin are separated at alkaline pH by ammonium sulfate extraction followed by ultrafiltration. Heme content is determined by absorption of the Soret band when the hemoprotein extract is visibly colored or more sensitively by its peroxidase activity when the extract has low color. The heme reacts with tertiary-butyl hydroperoxide and orthotolidine to generate a blue color. Hemoglobin content is correlated with myoglobin content and is related to aerobic capacity and blood flow to the tissue. Myoglobin content varied over 5 orders of magnitude up to 7 per cent of the weight of tissue, whereas hemoglobin content varied over 2 orders of magnitude up to 6 per cent of tissue weight. Myoglobin content is increased in species with high basal metabolic rate, high physical activity, prolonged diving capacity, fatigue resistance, and red muscle, whereas it is decreased in white muscle, iron-deficient animals, animals with sedentary lifestyles, and in animals and tissues with small fiber diameters such as avian or fish hearts.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
4F64220141EEFA587E0511354EDD7F7D074EE16F
Revue

Molecular and Cellular Biochemistry

Année de publication
1992
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.954
Sous-corpus
  • Mammiferes
  • Oiseaux
  • Poissons
Type de publication
Journal
ark:/67375/1BB-F6VN6DCQ-S
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