Nom du corpus

Corpus Systématique Animale

Titre du document

A high activity carbonic anhydrase isoenzyme (CA II) is present in mammalian spermatozoa

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00744
Auteur(s)
  • S. Parkkila 1
  • K. Kaunisto 1
  • S. Kellokumpu 1
  • H. Rajaniemi 1
Affiliation(s)
  • 1) Department of Anatomy, University of Oulu, Kajaanintie 52A, SF-90220, Oulu, Finland
Résumé

Human and rat spermatozoa were stained for different carbonic anhydrase (CA) isoenzymes using specific antisera to human CA I, II and VI in conjunction with the immunofluorescence technique. The spermatozoa of both species were found to contain only CA II, which was located principally in the postacrosomal region of the human spermatozoa and in the acrosomal cap region of the rat spermatozoa. The presence of CA II could be confirmed by immunoblotting, which revealed a 29 K polypeptide in both the human and rat spermatozoa. No CA I or VI-specific fluorescence could be detected in the spermatozoa of either species. The immunoblottings were also negative. The results show mammalian spermatozoa to contain the high activity carbonic anhydrase isoenzyme II. Its presence is probably linked to hydration of CO2 produced by active energy metabolism and thereby to the maintaining of an adequate intraspermatozoal bicarbonate concentration as required for the maintenance of sperm motility.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - vertebres: reproduction
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - General Agricultural and Biological Sciences
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Anatomy
Identifiant ISTEX
A708361D637AE6E8CF795AB602021A1DCBF17B71
Revue

Histochemistry

Année de publication
1991
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.273
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-F2RQ65SW-2
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