Nom du corpus

Corpus Systématique Animale

Titre du document

Characterization of intramuscular collagen in the mammalian left ventricle

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • left ventricular collagen
  • pepsin-solubilized collagen
  • collagen type distribution
  • hydroxyproline
  • polyacrylamide gel electrophoresis
Nom du fichier dans la ressource
Mammiferes_v2b_00843
Auteur(s)
  • Dr. I. Medugorac
Affiliation(s)
  • Physiologisches Institut II, Universität Tübingen, Gmelinstraße 5, 7400, Tübingen
Résumé

Left ventricular collagen of various mammals (cat, cow, dog, pig, and rat) was successively extracted with neutral salt, and dilute acid solutions, and limited pepsin digestion. The distribution of the various types of collagen molecules in pepsin-solubilized ventricular collagen was analyzed electrophoretically on SDS-polyacrylamide gels in the presence of 3.6 M urea. Yields of dilute-acid-soluble collagen were only 0.4 to 0.6% of the total ventricular collagen, and less than 0.1% with neutral salt solution. Approx. 55–90% of the total collagen was extracted by limited pepsin digestion. Disc patterns of pepsin-soluble collagen revealed the presence of dimeric and trimeric components, as well as higher-molecular-weight aggregates in all samples of nonreduced and reduced ventricular collagen. Taken together, these findings suggest the presence of an extensive interchain and intermolecular cross-linking network in left ventricular collagen. Comparison of electrophoretical disc gel patterns of reduced and nonreduced pepsin-solubilized collagen indicated that left ventricular collagen is heterogenous in nature, consisting of a mixture of type I and type III collagen. It was evident that primarily type I components occur in ventricular collagen. The components of type III collagen molecules occurred in all investigated left ventricles in varying and consistently appreciably lower amounts. The proportions of type III and type I collagen vary in left ventricular tissue of different species.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - clinical medicine
  • 3 - cardiovascular system & hematology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Physiology (medical)
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Cardiology and Cardiovascular Medicine
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Physiology
Catégories WoS
  • 1 - science ; 2 - cardiac & cardiovascular systems
Identifiant ISTEX
B44758B1FC54DF3150AFA08DFED500F6302D7899
Revue

Basic Research in Cardiology

Année de publication
1982
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.568
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-DLCJ43Z6-S
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