Nom du corpus

Corpus Systématique Animale

Titre du document

Development of a new method for obtaining osteoclasts from endosteal surfaces

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • osteoclast isolation
  • migratory osteoclasts
  • vitronectin-coated surfaces
  • osteoclast-like cell characterization
Nom du fichier dans la ressource
Oiseaux_v2b_01472
Auteur(s)
  • Lisa G. May 1
  • Carol V. Gay 2
Affiliation(s)
  • 1) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania
  • 2) Department of Poultry Science, The Pennsylvania State University, 469 North Frear Building, 16802, University Park, Pennsylvania
Résumé

Techniques for the isolation of ahhighly pure population of viable osteoclasts are limited. For this reason, we developed an isolation procedure that results in a high yield of osteoclast-like cells, up to 92% pure, from 3-wk-old chicken tibias. The unique feature of the method is the migration of cells from marrow-free endosteal surfaces to vitronectin-coated plates. The cells retain the osteoclast phenotype and remain viable in culture for a minimum of 1 wk. The cells were characterized and compared to two populations of authentic avian osteoclasts, which were isolated on the basis of association with fibronectin-coated plates. The cells contained substantial amounts of tartrate-resistant acid phosphatase. Alkaline phosphatase levels were negligible, suggesting little contamination by osteoblasts. Response to parathyroid hormone, dibutyryl cyclic adenosine monophosphate, calcitonin, acetazolamide, 17?-estradiol, and prostaglandin E2 was evident, as detected by measuring acid production. The vitronectin-associating cells contained numerous mitochondria, had the ability to resorb bone in anin vitro bone slice assay, and specifically bound biotinylated vitronectin. At 5 d of culture, the cells demonstrated marginal multinuclearity, having two to three nuclei. A large number (?1×106 cells/tibia) of viable cells that exhibit characteristics of authentic osteoclasts can be obtained by the method described. Potentially, this method could be applied to other species.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Developmental Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - developmental biology
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
7795A5E65C531838DF5B67F4EF407569F64B9704
Revue

In Vitro Cellular & Developmental Biology - Animal

Année de publication
1996
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.484
Sous-corpus
  • Oiseaux
Type de publication
Journal
ark:/67375/1BB-DJM0G6T5-V
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