Nom du corpus

Corpus Systématique Animale

Titre du document

Responses of channel catfish to xenobiotics: Induction and partial characterization of a mixed function oxygenase

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Poissons_v2b_003191
Auteur(s)
  • Sue W. Fingerman 1
  • Louise A. Brown 1
  • Melanie Lynn 1
  • Everett C. Short Jr. 1
Affiliation(s)
  • 1) Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, 74078, Stillwater, Oklahoma
Résumé

The effects of the environmental pollutant benzo(a)pyrene (BP) were assessed on the hepatic mixed function oxygenase system (MFO) in the channel catfish,Ictalurus punctatus. Initially, the optimum conditions were established for measuring hepatic MFO in this species of fish. Parameters investigated were divalent metal ion requirement,in vitro incubation temperature for optimum activity, pH for optimum activity and the time course for enzyme induction. A single dose of BP (100?g/kg body weight) given intraperitoneally to catfish kept in the laboratory at 19†C induced the hepatic MFO benzo(a)pyrene hydroxylase (BaPH) with maximum activity occurring 96 hr after the injection of BP. Cytochrome P450 assays were carried out at 24 hr and 48 hr after injection of BP. Hepatic cytochrome P450 and BaPH activity increased from 24 to 48 hr after the BP injection. In a temperature acclimation experiment, fish were maintained at 15†, 19†, and 26†C; assays for BaPH were conducted 24, 48, and 96 hr after an inoculation with BP. Induction occurred equally in all BP-injected fish by 24 hr; by 48 hr, induction was greatest in the fish kept at 26†C, least in the 15†C group. The fish kept at 19†C were intermediate. However, by 96 hr, induction had occurred to the same degree in all three experimental groups. BaPH activity was always greater in the BP-injected groups than in their respective control groups. Phenobarbital (75 mg/kg body weight) failed to cause an increase in hepatic dealkylating activities or cytochrome P450 concentration.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - toxicology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
Catégories Scopus
  • 1 - Physical Sciences ; 2 - Environmental Science ; 3 - Health, Toxicology and Mutagenesis
  • 1 - Physical Sciences ; 2 - Environmental Science ; 3 - Pollution
  • 1 - Life Sciences ; 2 - Pharmacology, Toxicology and Pharmaceutics ; 3 - Toxicology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - toxicology
  • 1 - science ; 2 - environmental sciences
Identifiant ISTEX
D18515C93A0891B62B4406F8D1C91C3992FCA10B
Revue

Archives of Environmental Contamination and Toxicology

Année de publication
1983
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.904
Sous-corpus
  • Poissons
Type de publication
Journal
ark:/67375/1BB-B0C1L7PB-8
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