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Corpus Systématique Animale

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Poly(ADP-ribose) polymerase activity in intact or permeabilized leukocytes from mammalian species of different longevity

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Springer (journals)
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Mots-clés d'auteur
  • Poly(ADP-ribose) polymerase
  • DNA repair
  • life span
  • mononuclear leukocytes
Nom du fichier dans la ressource
  • Alexander Bürkle 1
  • Marcus Müller 1
  • Ivo Wolf 1
  • Jan-Heiner Küpper 1
  • 1) Forschungsschwerpunkt “Angewandte Tumorvirologie”, Deutsches Krebsforschungszentrum, Postfach 10 19 49, D-69009, Heidelberg, Germany

Poly(ADP-ribosyl)ation is a eukaryotic posttranslational protein modification catalyzed by poly(ADP-ribose) polymerase (PARP), a highly conserved nuclear enzyme which uses NAD as substrate. We have previously tested PARP activity in permeabilized mononuclear blood cells (MNC) from 13 mammalian species as a function of the species-specific life span. A direct and maximal stimulus of PARP activation was provided by including saturating amounts of a double-stranded ollgonucleotide in the PARP-reaction buffer. The data yielded a strong positive correlation between PARP activities and the species' maximal life spans (r=0.84; p?0.001). Here, we investigated the formation of poly(ADP-ribose) inliving MNC from two mammalian species with widely differing longevity (rat and man) by immunofluorescence detection of poly(ADP-ribose). The fraction of positive cells was recorded, following ?-irradiation of intact MNC, as a semiquantitative estimation of poly(ADP-ribose) formation. Human samples displayed a significantly higher percentage of positivity than did those from rats, consistent with our previous results on permeabilized cells. While rat MNC had a higher NAD content than human MNC, the number of radiation-induced DNA strand breaks was not significantly different in the two species. Since poly(ADP-ribosyl)ation is apparently involved in DNA repair and the cellular recovery from DNA damage, we speculate that the higher poly(ADP-ribosyl)ation capacity of long-lived species might more efficiently help to slow down the accumulation of unrepaired DNA damage and of genetic alterations, as compared with short-lived species. (Mol Cell Biochem138: 85–90, 1994)

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
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  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
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Molecular and Cellular Biochemistry

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  • Mammiferes
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