Nom du corpus

Corpus Systématique Animale

Titre du document

Demonstration of the neural crest origin of type I (APUD) cells in the avian carotid body, using a cytochemical marker system

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Oiseaux_v2b_02968
Auteur(s)
  • A. G. E. Pearse 1,2
  • J. M. Polak 1,2
  • F. W. D. Rost 1,2
  • J. Fontaine 1,2
  • C. Le Lièvre 1,2
  • N. Le Douarin 1,2
Affiliation(s)
  • 1) Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
  • 2) Laboratoire d'Embryologie de la Faculté des Sciences de Nantes, Cedex Nantes, France
Résumé

The biogenic amines present in the carotid body Type 1 cells of two avian species (Japanese quail and chicken) were identified, by microspectrofluorometry of formaldehyde-induced fluorescence, as dopamine and 5-hydroxytryptamine respectively. These and other cytochemical properties establish the cells as members of the APUD series. Grafts of the neural rhombencephalic primordium from 6 to 10-somite quail embryos were implanted in the appropriate region of chick embryos of the same age. After up to 11 days incubation the carotid bodies of the host were freeze-dried and treated with hot formaldehyde vapour. The carotid body Type 1 cells in the chick host were identified, by the presence of dopamine and the absence of 5-HT, as cells from the quail neural crest. The dopamine phenotype in cells of quail origin thus provides a cytochemical marker which may be used for other allograft experiments. The present work confirms earlier findings, using a biological (nuclear chromatin) marker, which showed the avian carotid body to be of neural crest origin.

Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Health Sciences ; 2 - Health Professions ; 3 - Medical Laboratory Technology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Histology
Identifiant ISTEX
5695741192FE4926421065981716B8786EBFB8A3
Revue

Histochemie

Année de publication
1973
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.98
Sous-corpus
  • Oiseaux
Type de publication
Journal
ark:/67375/1BB-9FD8V9RG-0
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