Nom du corpus

Corpus Systématique Animale

Titre du document

d -ephedrinephosphate, DEP: A new substrate with specificity for prostatic acid phosphatase (PAP)

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Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00687
Auteur(s)
  • Buddha D. Paul 1,2
  • José A. Serrano 1,2
  • Hannah L. Wasserkrug 1,2
  • Anna A. Serrano 1,2
  • Arnold M. Seligman 1,2
Affiliation(s)
  • 1) Department of Research Oncology and Cell Biology, Sinai Hospital of Baltimore, Inc., 21215, Baltimore, MD, USA
  • 2) Department of Surgery, The Johns Hopkins University School of Medicine, 21205, Baltimore, MD, USA
Résumé

This report describes the synthesis and physical (including spectral) properties of a new substrate, d-ephedrinephosphate, DEP1, which is histochemically highly specific for a secreted non-lysosomal prostatic acid phosphatase, PAP. This specificity is in contrast to other substrates which are nonspecific, i.e., which demonstrate acid phosphatases that originate from various cell types and are mainly lysosomal. When this substrate is used for light and electron microscopic histochemistry in a modified Gomori medium, PAP is demonstrated mainly in secretory granules and in the Golgi apparatus (and its related vacuoles) of prostatic epithelial cells of several species of mammals including man. This corroborates our previous suggestion that PAP is not a lysosomal enzyme as are many of the other acid phosphatases. This high degree of specificity of DEP for PAP supports the usefulness of this compound in the histochemical and biochemical characterization of PAP, and in the diagnosis of localized or disseminated prostatic disease.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - General Agricultural and Biological Sciences
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Anatomy
Identifiant ISTEX
8E116A3D7A70B87863BCAC40A961F52AFB5A8C56
Revue

Histochemistry

Année de publication
1978
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.797
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-7T9DHDLS-C
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