Laboratory maintenance of Monanema globulosa , a rodent filaria with skin-dwelling microfilariae
- Immunoparasitology Unit, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Post Office, 3050, Victoria, Australia
The life-cycle ofMonanema globulosa (Nematoda: Filarioidea) was established in the laboratory in the hard tick,haemaphysalis leachi s.l. and the striped mouse,Lemniscomys striatus. Of eight species or strains of commercially available rodents, only the jirdMeriones unguiculatus was susceptible to experimental infections. 64% of laboratory-bred striped mice and 63% of jirds developed patent infections following the subcutaneous inoculation of infective larvae. Striped mice were also infected by exposure to the bites of infected ticks, but larvae failed to develop following intravenous, intraperitoneal, or oral routes of administration. There was a significant difference in the prepatent period in jirds (mean, 74 days) and striped mice (mean, 88 days) and microfilariae persisted in the skin for longer in the former host species (means, 370 and 175 days respectively). The number of infective larvae administered was positively correlated with the length of the patent infection, but not with densities of microfilariae in the ears which were similar in both species of rodents (range, 3–19 mf/mg). Single or repeated inoculations of infective larvae resulted in patent infections of similar duration and skin densities of microfilariae. However, worm recoveries were low from most experimentally infected rodents and this appeared to be associated with an age-related resistance to developing larvae. It is concluded thatM. globulosa is a relatively difficult filaria to maintain in the laboratory, but offers considerable promise as a rodent model for onchocerciasis if heavier experimental infections can be produced.
- 1 - sciences appliquees, technologies et medecines
- 2 - sciences biologiques et medicales
- 3 - sciences biologiques fondamentales et appliquees. psychologie