Nom du corpus

Corpus Systématique Animale

Titre du document

Mapping and characterization of Paracentrotus lividus mitochondrial transcripts: multiple and overlapping transcription units

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • RNA mapping
  • RNA processing
  • Mitochondrial transcription
Nom du fichier dans la ressource
Echinodermes_v2b_0392
Auteur(s)
  • Palmiro Cantatore 1
  • Marina Roberti 1
  • Paola Loguercio Polosa 1
  • Anna Mustich 1
  • Maria N. Gadaleta 1
Affiliation(s)
  • 1) Department of Biochemistry and Molecular Biology, University of Bari and C.S.M.M.E., C.N.R. Traversa ReDavid 200, I-70126, Bari, Italy
Résumé

This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5? and 3? termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3? ends of the two rRNAs (12S and 16S) overlap with the downstream transcripts (tRNAGlu and CoI mRNA) by 5 and 10 nt respectively. The 132nt non-coding region is extensively transcribed: in particular it contains a 124nt RNA and the 5? end of a possible precursor of 13 clustered tRNAs. This latter overlaps by 7nt with the 3? end of the 124nt RNA. In addition to the mature RNAs, 32 high molecular weight RNAs, which are probably the precursors of the smaller more abundant mature species, were detected by Northern blotting. The mapping of these transcripts indicates that they are processed at the level of tRNA or tRNA-like sequences and suggests the existence of two transcription initiation sites upstream of the ND1 and the cytochrome b genes respectively. In the light of these results it appears that P. lividus mitochondrial DNA transcription takes place via multiple and probably overlapping transcription units. Moreover, the wide variation in the steady-state levels of the mature mRNAs indicates that sea urchin mitochondrial DNA expression is also regulated at the level of RNA decay.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - microbiology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
Identifiant ISTEX
4F4774E9489D3FF038F8E40279C621E0C3566687
Revue

Current Genetics

Année de publication
1990
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.604
Sous-corpus
  • Echinodermes
Type de publication
Journal
ark:/67375/1BB-7MSHF0RH-9
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