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Corpus Systématique Animale

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Evolution of the single copy ? A-crystallin gene: differently sized mRNAs of mammals and birds show homology in their 3? non-coding regions

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Mammiferes_v2b_00469, Oiseaux_v2b_00535, Poissons_v2b_000896, Reptiles_v2b_00143
  • H. J. Dodemont 1
  • W. J. Quax 1
  • J. G. G. Schoenmakers 2
  • H. Bloemendal 1
  • 1) Department of Biochemistry, University of Nijmegen, Geert Grooteplein N21, 6525 EZ, Nijmegen, The Netherlands
  • 2) Department of Molecular Biology, University of Nijmegen, Toernooiveld, 6525 ED, Nijmegen, The Netherlands

?A-Crystallin, a major structural polypeptide of the vertebrate eye lens, is evolutionarily highly conserved. We have analyzed the corresponding nucleic acid sequences in both genomic DNA digests as well as in lens cytoplasmic RNA preparations from a wide variety of vertebrates by blot hybridization with cloned rat ?A2-crystallin cDNA probes. The probes are not able to hybridize under any conditions to RNA and DNA derived from fishes and amphibia, but do show substantial homology with the sequences of mammals, birds and reptiles. The ?A-crystallin gene, which has been isolated from a hamster gene library occurs only once in the haploid genome. Coding and 3?-untranslated regions of ?A2-crystallin mRNA are conserved among all mammals and birds examined. However, the regions comprising the conserved sequences are differently represented in the ultimate mRNA. The ?A2-mRNA 3?-non-coding regions of reptiles and birds are 300–550 bases longer than those of mammals. Some rodents produce next to the ?A2-mRNA another messenger that encodes the ?Alns-polypeptide possessing an insertion of 22/23 amino acid residues between positions 63 and 64 of the ?A2-polypeptide chain. ?A2 and ?Alns-mRNA are generated from a single gene as major and minor species, respectively, in a proportion which is similar to the ratio of the polypeptides foundin vivo andin vitro. The size heterogeneity of the ?A2-mRNA from most mammals examined is due to the variable size of the poly(A) tail.

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  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
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  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
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  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
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  • 1 - science ; 2 - biochemistry & molecular biology
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Molecular Biology Reports

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  • Mammiferes
  • Oiseaux
  • Poissons
  • Reptiles
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