- 1) Department of Life Sciences, University of London Goldsmiths' College, Rachel McMillan Building, Creek Road, SE8 3BU, London, UK
- 2) Department of Zoology, University of Auckland, Private Bag, Auckland, New Zealand
Proteins from Antarctic fish are less stable at high temperatures than those from fish from lower latitudes. Investigations into the thermostability of haemoglobins from a range of Antarctic teleosts have been carried out for comparison with data from temperate species. Haemoglobin concentrations following periods of heating at 50°C were analysed spectrophotometrically and the time taken for 50% denaturation (t50%) determined. The effects of pH and salt concentrations were also examined. With the exception of that of Rhigophila dearborni, the haemoglobins were found to be relatively unstable with t50% values ranging from 7.7 to 29.9 min at pH 7. All haemoglobins became less stable on addition of KCl but the effect of pH was variable. Freezing had no effect on the stability of haemoglobin from Dissostichus mawsoni. The thermostability of haemoglobin from a temperate nototheniid, Notothenia angustata, was within the range displayed by its antarctic relatives and it would seem that in general the differences between genera are as great as those between Antarctic and temperate species as a whole.
- 1 - sciences appliquees, technologies et medecines
- 2 - sciences biologiques et medicales
- 3 - sciences biologiques fondamentales et appliquees. psychologie
- 4 - vertebres: zoologie generale, morphologie, phylogenese, systematique, cytogenetique, repartition geographique
- 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - General Agricultural and Biological Sciences