Nom du corpus

Corpus Systématique Animale

Titre du document

Cytochrome P-450 monooxygenase system and benzo(a)pyrene metabolism in echinoderms

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Echinodermes_v2b_0032, Mollusques_v2b_00067
Auteur(s)
  • P. J. den Besten 1
  • H. J. Herwig 1
  • E. G. van Donselaar 1
  • D. R. Livingstone 2
Affiliation(s)
  • 1) Research Group for Aquatic Toxicology, University of Utrecht, P.O. Box 80058, N-3508, Utrecht, TB, The Netherlands
  • 2) Plymouth Marine Laboratory, Citadel Hill, PL1 2PB, Plymouth, UK
Résumé

A comparative study on mixed-function oxygenase (MFO) systems was carried out on four echinoderm species: the asteroidsAsterias rubens andMarthasterias glacialis, a holothurianHolothuria forskali and an echinoidEchinus esculentus. Cytochromes P-450 andb 5 and the MFO system-associated NADH-ferricyanide reductases NADH-cytochromec reductase, NADPH-cytochromec reductase and benzo(a)pyrene (BP) hydroxylase activities were present in microsomal fractions of pyloric caeca ofA rubens andM. glacialis and in the haemal plexus ofH. forskali. In contrast, cytochrome P-450 and BP hydroxylase activity were not detectable in the gonads ofE. esculentus. The tissue and subcellular distribution of the MFO system was studied inA. rubens. MFO system components were found in the stomachs and gonads, although detection of cytochrome P-450 in the latter tissue was difficult. Sex-related differences were not significant. The contents of the MFO system components and BP hydroxylase activities were highest in the microsomal (100 000 ×g) fractions, but MFO system components were also found in the mitochondrial (12 000 ×g) and cytosolic fractions. The BP hydroxylase activity in pyloric caeca microsomes ofA. rubens was NADPH-dependent and was inhibited by several agents known to be inhibitors of vertebrate cytochromes P-450. In the former respect, the characteristics of the MFO system were more like those of vertebrates and crustaceans than that of molluscs.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - marine biology & hydrobiology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - ethologie animale
Catégories Scopus
  • 1 - Physical Sciences ; 2 - Environmental Science ; 3 - Ecology
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Aquatic Science
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Ecology, Evolution, Behavior and Systematics
Catégories WoS
  • 1 - science ; 2 - marine & freshwater biology
Identifiant ISTEX
2788F37CC9B3BCC35E4570B64DC0123ABF46D743
Revue

Marine Biology

Année de publication
1990
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.46
Sous-corpus
  • Echinodermes
  • Mollusques
Type de publication
Journal
ark:/67375/1BB-5HFMVXJL-Q
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