Nom du corpus

Corpus Systématique Animale

Titre du document

Reduction of ?-thiopyruvic acid by lactate dehydrogenase: a kinetic study

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_01017
Auteur(s)
  • B. Pensa 1
  • M. Costa 1
  • A. Colosimo 2
  • D. Cavallini 1
Affiliation(s)
  • 1) Istituto di Chimica Biologica, Universitá di Roma e Centro di Biologia Molecolare del C.N.R., Roma, Italia
  • 2) Istituto di Chimica, Facoltá di Medicina, Università di Roma e Centro di Biologia Molecolare del C.N.R., Roma, Italia
Résumé

In this paper a steady-state kinetic study on the system lactate dehydrogenase-?-thiopyruvate-?-thiolactate is presented and the possible mechanistic and physiological implications are discussed. At pH 7.4 the equilibrium between ?-thiopyruvate and ?-thiolactate, in the presence of NADH and lactate dehydrogenase is largerly shifted towards the formation of ?-thiolactate as in the case of pyruvate and lactate. This can be relevant in connection with the mixed disulfide between cysteine and ?-thiolactate that is observed to be present in the mammalian body fluids. The catalytic mechanism is of the bi-bi compulsory order type, and rapid equilibrium conditions for the binding of the first substrate (NADH) are shown to apply. A complex inhibition pattern of inhibitions by both substrates, however, prevents simple suggestions about the nature of the dead-end species involved.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
35B2B7294CA4F6EED6C3D891D1AE771E64F68F23
Revue

Molecular and Cellular Biochemistry

Année de publication
1982
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.077
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-5251BD8S-2
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