Nom du corpus

Corpus Systématique Animale

Titre du document

Temperature-dependent deacylation of molecular species of phosphatidylcholine by microsomal phospholipase A2 of thermally acclimated rainbow trout, Salmo gairdneri 1

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Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Poissons_v2b_000260
Auteur(s)
  • N. P. Neas 1
  • J. R. Hazel 1
Affiliation(s)
  • 1) Department of Zoology, Arizona State University, 85287, Tempe, AZ
Résumé

Using the ratios of kinetic parameters, V/Km, the deacylation of different molecular species of 1-palmitoyl,2-acyl phosphatidylcholine via microsomal phospholipase A2 (PLA2) was studied in liver tissue of thermally acclimated rainbow trout (Salmo gairdneri). In general, PLA2 from fish acclimated to cold temperatures showed an order of preference for the acyl moieties of 18?1>18?1>18?2>18?0. Trout acclimated to warm temperatures generally preferred 18?0 PC, but the actual order of preference depended on the temperature of the assays and the presence of endogenous lipids in the enzyme preparation. At 5 C, the particulate (microsomal) enzyme preferred 18?0>18?2>18?1, but a lipid-free preparation of the enzyme preferred 18?2>18?0>18?1. At 20 C, particulate enzyme preferred 18?1>18?0>18?2 but purified enzyme preferred 18?0>18?2>18?1. Thus, assay temperature and the presence of microsomal lipids had a greater effect on PLA2 from fish acclimated to warm temperatures than fish acclimated to cold temperatures. The substrate preference of PLA2 is discussed with reference to the previously observed changes in membrane fatty acid composition that occur with thermal acclimation in rainbow trout.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - nutrition & dietetics
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Physical Sciences ; 2 - Chemistry ; 3 - Organic Chemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - nutrition & dietetics
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
540567F616925F8182BD40804C80DAB61BF2B252
Revue

Lipids

Année de publication
1984
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.863
Sous-corpus
  • Poissons
Type de publication
Journal
ark:/67375/1BB-51H1ZHVV-3
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