Nom du corpus

Corpus Systématique Animale

Titre du document

Preparation and characterization of monoclonal antibodies to human hexokinase type I

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • hexokinase
  • monoclonal antibodies
  • comparative studies
Nom du fichier dans la ressource
Mammiferes_v2b_01086
Auteur(s)
  • Laura Chiarantini 1
  • Giordano Serafini 1
  • Vilberto Stocchi 1
  • Mauro Magnani 1
Affiliation(s)
  • 1) Istituto di Chimica Biologica ‘G. Fornaini’, Università degli Studi, Via Saffi, 2-61029, Urbino, Italy
Résumé

1. Three different immunization protocols and several screening procedures were used to prepare seven mouse monoclonal antibodies to human placenta hexokinase type I. None of these monoclonals were able to recognize the native enzyme but all detected hexokinase when adsorbed onto polystyrene plates or on immunoblots after SDS/polyacrylamide-gel electrophoresis. 2. All seven monoclonals recognize the two different subtypes of human hexokinase I equally well. Limited tryptic digestion of hexokinase followed by Western blotting and immunodetection show that these monoclonals recognize epitopes that lie in different tryptic peptides. 3. Comparative ELISA studies showed that human hexokinase types I and II have great immunological similarities while hexokinase I from different mammalian species and yeast hexokinase are recognized with different affinities.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Clinical Biochemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
73CEE4176617662DDB4C5FB93E87D3D33C664F00
Revue

Molecular and Cellular Biochemistry

Année de publication
1990
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.142
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-4WRTNPRK-L
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