Nom du corpus

Corpus Systématique Animale

Titre du document

Isolation and identification of novel sulfated and nonsulfated oligosialyl glycosphingolipids from sea urchin sperm

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • oligosialoglycosphingolipid
  • sulfated sialic acid
  • sea urchin sperm
  • C:M : chloroform:methanol
  • FAB-MS : fast atom bombardment mass spectrometry
  • GLC : gas-liquid chromatography
  • GSL : glycosphingolipid
  • HPTLC : high performance thin-layer chromatography
  • Neu5Ac8HSO3 or HSO3 ? Neu5Ac : 5-N-acetyl-8-O-sulforylneuraminic acid
  • Neu5Gc9HSO3 : 5-N-glycolyl-9-O-sulforylneuraminic acid
  • NMR : nuclear magnetic resonance
  • PB : sodium phosphate buffer, pH 7.2
  • Sia : sialic acid
  • HSO3 ? Sia : sulfated sialic acid
  • polySia : polysialic acid
  • TFA : trifluoroacetic acid
Nom du fichier dans la ressource
Echinodermes_v2b_0308
Auteur(s)
  • Takeshi Ijuin 1
  • Ken Kitajima 1
  • Yu Song 1
  • Shinobu Kitazume 1
  • Sadako Inoue 2
  • Stuart M. Haslam 3
  • Howard R. Morris 3
  • Anne Dell 3
  • Yasuo Inoue 1
Affiliation(s)
  • 1) Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo-7, 113, Tokyo, Japan
  • 2) School of Pharmaceutical Sciences, Showa University, Hatanodai-1, 142, Tokyo, Japan
  • 3) Department of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AZ, London, UK
Résumé

Novel sulfated and nonsulfated oligosialyglycosphingolipids were isolated from sperm of the sea urchin,Hemicentrotus pulcherrimus, and their structures were established as follows: ±HSO3?Neu5Ac?2?(8Neu5Ac?2?)n?6G1c?1?1´Cer, wheren=0, 1, 2, 3. This provides the first evidence for the natural occurrence of a tetrasialic acid structure in glycosphingolipids. The finding of sulfated oligosialyl chains is especially noteworthy in that the sulfate group exclusively resides on the C-8 of the nonreducing terminal residues of oligo/polysialyl chains and that sulfation appears to be a termination signal for elongation of oligosialyl chains. Sulfation at the nonreducing terminal Neu5Ac residues of oligosialyl chains was also found to facilitate the formation of an inter-residue lactone between the carboxyl group at the nonreducing terminal sulfated Neu5Ac and the hydroxyl group at C-9 of the penultimate Neu5Ac residue. The long chain base was 4-hydroxysphinganine (t18:0) and the major fatty acid species were identified as C20:1, C21:1, and C22:1.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - biochimie analytique, structurale et metabolique
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
DB57094B65BB76610934B0FAF9E5FD8998191496
Revue

Glycoconjugate Journal

Année de publication
1996
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.752
Sous-corpus
  • Echinodermes
Type de publication
Journal
ark:/67375/1BB-4Q0FR1MV-G
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