Nom du corpus

Corpus Systématique Animale

Titre du document

Conservation of repetitive DNA sequences in deer species studied by Southern blot transfer

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Chromosome evolution
  • Restriction enzymes
  • Nick translation
  • DNA blot transfer
  • Deer species
  • Repetitive DNA
Nom du fichier dans la ressource
Mammiferes_v2b_02739
Auteur(s)
  • A. Lima-de-Faria 1
  • U. Arnason 2
  • B. Widegren 2
  • J. Essen-Möller 1
  • M. Isaksson 1
  • E. Olsson 1
  • H. Jaworska 1
Affiliation(s)
  • 1) Institute of Molecular Cytogenetics, University of Lund, Tornavägen 13, S-223 63, Lund, Sweden
  • 2) Institute of Genetics, University of Lund, Solvegatan 29, S-223 63, Lund, Sweden
Résumé

The Cervidae show one of the largest variations in chromosome number found within a mammalian family. The five species of the deer family which are the subject of this study vary in chromosome number from 2n=70 to 2n=6. Digestion with the restriction enzymes EcoRI, HpaII, HaeIII and MspI reveals that there is a series of highly repetitive sequences forming similar band patterns in the different species. To obtain information on the degree of homology among these conserved sequences we isolated a HpaII restriction fragment of approximately 990 base pairs from reindeer DNA. This DNA sequence was32P-labelled and hybridized by the Southern blot technique to DNAs cleaved with HpaII and HaeIII from the reindeer and four other Cervidae species. Hybridization to specific restriction fragments was recorded in all species. The patterns of hybridization showed a higher degree of similarity between reindeer, elk and roe deer than between reindeer and the Asiatic species (fallow deer and muntjac). Homologies are still present between the highly repetitive sequences of the five species despite the drastic reorganization that led to a change in chromosome number from 6 to 70.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - evolutionary biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Ecology, Evolution, Behavior and Systematics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - evolutionary biology
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
8DF5403BB9F604CD81879CF32F53B07F188D3B31
Revue

Journal of Molecular Evolution

Année de publication
1984
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.112
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-4N96M58G-J
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