Nom du corpus

Corpus Systématique Animale

Titre du document

Ultrastructural observations of amorphous bone mineral in avian bone

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • Amorphous
  • Bone mineral
  • Apatite
  • Electron microscopy
Nom du fichier dans la ressource
Oiseaux_v2b_02709
Auteur(s)
  • A. L. Miller 1
  • Dr. H. Schraer 1
Affiliation(s)
  • 1) Biophysics Department, The Pennsylvania State University, University Park, 618 Life Sciences Building, 16802, University Park, PA, USA
Résumé

Mineral from medullary bone of three avian species was examined with the electron microscope in order to clarify the ultrastructure of amorphous bone mineral (ABM) in a mineralized tissue. Powders from freeze-dried bone revealed bone mineral with morphology and behavior identical to synthetic amorphous calcium phosphate (ACP). These powders exhibited spherically shaped particles 180–400 Å in diameter with uniform electron density when viewed at low bem intensity. Thin sections of embedded freeze-dried bone also revealed spherically shaped particles 100–350 Å in diameter with electron beam sensitivity characteristic of ACP. Regions of bone mineral with irregular outline and morphology were observed which closely resemble the discoidal form of synthetic ACP. More electron-dense spherical particles (150 Å in diameter) could be seen budding from these regions. Some of these buds exhibited electronlucent centers characteristics of AMB. The inorganic nature of these features of bone mineral was confirmed using ultramicroincineration. Preliminary exploration of a freeze-substitution technique showed spherical bone mineral particles which were similar in morphology to those observed in freeze-dried samples. A limited degree of preservation of cellular material was observed using this freeze-substitution technique.

Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Endocrinology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Identifiant ISTEX
C933B9CD0EE8B961905BEED284845B8B1EF625CE
Revue

Calcified Tissue Research

Année de publication
1975
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.208
Sous-corpus
  • Oiseaux
Type de publication
Journal
ark:/67375/1BB-47KF0WKN-N
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