Nom du corpus

Corpus Systématique Animale

Titre du document

Duplication of the MHC-linked Xenopus complement factor B gene

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Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_02598
Auteur(s)
  • Yoichi Kato 1
  • Luisa Salter-Cid 2
  • Martin F. Flajnik 2
  • Chisato Namikawa 1
  • Makoto Sasaki 1
  • Masaru Nonaka 1
Affiliation(s)
  • 1) Department of Biochemistry, Nagoya City University Medical School, Mizuho-Ku, 467, Nagoya, Japan
  • 2) Department of Microbiology and Immunology, University of Miami School of Medicine, 33101, Miami, FL, USA
Résumé

We have previously reported the molecular cloning of the mammalian major histocompatibility complex (MHC) class III gene, complement factor B (Bf) from Xenopus laevis, and linkage of the gene to the frog MHC. Here, we estimated the copy number of the Xenopus Bf gene by genomic Southern blotting analysis and demonstrated that Xenopus laevis has two copies of the Bf gene. Both genes co-segregated with the MHC-linked HSP70 genes among 19 offspring of an f/r × f/r cross, indicating a close linkage of the two Bf genes to the frog MHC. Both genes are transcribed and contain open reading frames. When compared with the previously determined cDNA sequence (Xenopus Bf A), the predicted amino acid sequence of the second cDNA species (Xenopus Bf B) shows 82% overall identity. Polymerase chain reaction analysis indicated that all of the partially inbred frogs with the f, r, g, and j MHC haplotypes, as well as 12 outbred frogs tested have both Bf genes, suggesting that the duplicated Bf genes are stable genetic traits in Xenopus laevis.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - clinical medicine
  • 3 - immunology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Immunology and Microbiology ; 3 - Immunology
Catégories WoS
  • 1 - science ; 2 - immunology
  • 1 - science ; 2 - genetics & heredity
Identifiant ISTEX
1B111B2C00AE65DDFC929F1290A72475BE938DF5
Revue

Immunogenetics

Année de publication
1995
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.088
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-44S34SJN-2
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