Nom du corpus

Corpus Systématique Animale

Titre du document

Identification and characterization of T-cell antigen receptor-related genes in phylogenetically diverse vertebrate species

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Poissons_v2b_005235
Auteur(s)
  • Jonathan P. Rast 1
  • Robert N. Haire 1
  • Ronda T. Litman 1
  • Susan Pross 2
  • Gary W. Litman 1
Affiliation(s)
  • 1) All Children's Hospital, University of South Florida, 801 Sixth Street South, 33701, St. Petersburg, FL, USA
  • 2) University of South Florida, 12901 Bruce B. Downs Boulevard, 33612, Tampa, FL, USA
Résumé

Characterization of the structure, multiplicity, organization, and cell lineage-specific expression of T-cell receptor (TCR) genes of nonmammalian vertebrate species is central to the understanding of the evolutionary origins of rearranging genes of the vertebrate immune system. We recently described a polymerase chain reaction (PCR) strategy that relies on short sequence similarities shared by nearly all vertebrate TCR and immunoglobulin (Ig) variable (V) regions and have used this approach to isolate a TCR beta (TCRB) homolog from a cartilaginous fish. Using these short PCR products as probes in spleen cDNA and genomic libraries, we were able to isolate a variety of unique TCR and TCR-like genes. Here we report the identification and characterization of a chicken TCR gamma (TCRG) homolog, apparent Xenopus and pufferfish TCR alpha (TCRA) homologs, and two horned shark TCR delta (TCRD)-like genes. In addition, we have identified what could be a novel representative of the Ig gene super-family in the pufferfish. This method of using short, minimally degenerate PCR primers should speed progress in the phylogenetic investigations of the TCR and related genes and lend important insights into both the origins and functions of these unique gene systems.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - clinical medicine
  • 3 - immunology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - genetique des eucaryotes. evolution biologique et moleculaire
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Immunology and Microbiology ; 3 - Immunology
Catégories WoS
  • 1 - science ; 2 - immunology
  • 1 - science ; 2 - genetics & heredity
Identifiant ISTEX
53665EC60EE5AAC1E5BC22DB33CF19D00A3529F1
Revue

Immunogenetics

Année de publication
1995
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.304
Sous-corpus
  • Poissons
Type de publication
Journal
ark:/67375/1BB-43QXPTCN-B
Powered by Lodex 9.3.8