Nom du corpus

Corpus Systématique Animale

Titre du document

Different resistance of mammalian red blood cells to hemolysis by bile salts

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00431
Auteur(s)
  • G. Salvioli 1
  • E. Gaetti 1
  • R. Panini 1
  • R. Lugli 1
  • J. M. Pradelli 1
Affiliation(s)
  • 1) Geriatrics and Gerontology, University of Modena, Ospedale Estense, Viale Vittorio Veneto 9, 41100, Modena, Italy
Résumé

To evaluate why hemolysis of red blood cells (RBC) by bile acids varies in different mammalian species, we determined the mean corpuscular volume (MCV), lipid content and the concentrations of the conjugates of deoxycholate and of NaCl inducing 50% hemolysis of RBC from healthy humans, pigs, horses, cows, sheep and jaundiced humans. A volume of 0.05 mL of washed RBC at 1% hematocrit, which has the same lipid content but different phospholipid composition and number of erythrocytes (owing to the variable MCV), was incubated in taurodeoxycholate (TDC) solution (0–5 mM) to determine the TDC concentration inducing 50% hemolysis (TDC50). The TDC50 was highest in RBC of sheep and decreased within the series sheep>pig>cow>horse>healthy human>jaundiced human, which have generally increasing MCV. The osmotic resistance followed an inverse order, with jaundiced human >healthy human>horse>cow>pig>sheep. Although we found no correlation between the TDC50 and phospholipid composition of the erythrocytes tested, the extent of bile salt-induced hemolysis seemed to depend on both the MCV and the number of erythrocytes in the incubation medium.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - nutrition & dietetics
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
  • 4 - gastroenterologie. foie. pancreas. abdomen
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Physical Sciences ; 2 - Chemistry ; 3 - Organic Chemistry
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - nutrition & dietetics
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
49AFA817380BAEFDA1457716A5791B732972AD80
Revue

Lipids

Année de publication
1993
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
7.185
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-3KTDFH2K-5
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