Nom du corpus

Corpus Systématique Animale

Titre du document

In situ methylation of insect chromosomes with methylase HpaII

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Echinodermes_v2b_0310, Insectes_v2b_01588
Auteur(s)
  • C. Sentís 1
  • J. Santos 1
  • J. Fernández-Piqueras 1
Affiliation(s)
  • 1) Departamento de Biología, Unidad de Genética, Facultad de Ciencias, Mod. C-XV, Universidad Autónoma de Madrid, E-28049, Madrid, Spain
Résumé

A method of in situ DNA methylation with the prokaryotic methylase HpaII has been developed on fixed mitotic and meiotic chromosomes of the insect species Baetica ustulata. Incorporation of methyl groups into the chromosomal DNA is revealed by autoradiography using a labelled substrate and by its ability to prevent endonuclease digestion. The method allows direct visualization of clusters of methylatable CCGG sites. The distribution of these clusters in the chromosome complement of Baetica shows two separate domains of heterochromatic DNA which differ in their methylation patterns. Each is distributed at equivalent locations in both homologous and nonhomologous chromosomes. The existence of two compartments, one methylated and the other unmethylated, in the heterochromatic DNA could be interpreted as a remnant of the ancestral echinoderm-like pattern of methylation.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - developmental biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Genetics(clinical)
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
77BD5A270169F93F647E5A72B70A06BE8B9D5F97
Revue

Chromosoma

Année de publication
1989
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.807
Sous-corpus
  • Echinodermes
  • Insectes
Type de publication
Journal
ark:/67375/1BB-3CFF9WZV-B
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