Nom du corpus

Corpus Systématique Animale

Titre du document

An in vitro , serum-free organ culture technique for the study of development and growth of the dermal skeleton in fish

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • fish
  • skeleton
  • development
  • organ culture
  • serum free
Nom du fichier dans la ressource
Mammiferes_v2b_02143, Poissons_v2b_004143
Auteur(s)
  • Joseph T. M. Koumans 1
  • Jean-yves Sire 1
Affiliation(s)
  • 1) URA CNRS 1137, Laboratoire d’Anatomie comparée, Université Paris 7, Case 7077, Equipe “Formations Squelettiques”, 2, place Jussieu, 75251, Paris cedex 05, France
Résumé

To develop a serum-free, chemically definedin vitro organ culture system enabling the study of epithelial-mesenchymal interactions in development and growth of fish dermal skeleton, we investigatedin vitro continuation of scale regeneration in the cichlid fishHemichromis bimaculatus. The culture medium in our system is based on Leibovitz medium (L-15) supplemented with vitamin C, additional amino acids and HEPES. With this basis medium, we examined the effects of all trans-retinoic acid, dexamethasone, and prostaglandin-E2 (PG-E2), factors known to exert an effect on development and growth of teeth and bone in mammalian culture systems, on thein vitro regeneration of scales. These effects were compared with those obtained by supplementation of the basis medium with newborn and fetal calf serum. To evaluate our culture system, the medium that allowed to mimick in the best possible way thein vivo regeneration of scales (i.e., the basis medium plus dexamethasone and PG-E2) was also tested on thein vitro development of teeth in the same fish species. Our serum-free, chemically defined organ culture system enablesin vitro development and growth of both scales and teeth. With this model culture system, it is possible to evaluate thein vitro effects of hormones, growth factors, and other substances on growth and development of dermal skeleton in fish.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Cell Biology
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Developmental Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - developmental biology
  • 1 - science ; 2 - cell biology
Identifiant ISTEX
5C247298D7892685A27AF2C0AF99BF413FB16A1D
Revue

In Vitro Cellular & Developmental Biology - Animal

Année de publication
1996
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.472
Sous-corpus
  • Mammiferes
  • Poissons
Type de publication
Journal
ark:/67375/1BB-32ZNDL5V-3
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