Nom du corpus

Corpus Systématique Animale

Titre du document

Aldehyde dehydrogenase (ALDH) isozymes in the gray short-tailed opossum ( Monodelphis domestica ): Tissue and subcellular distribution and biochemical genetics of ALDH3

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Mots-clés d'auteur
  • aldehyde dehydrogenase
  • isozymes
  • opossum
  • genetics
Nom du fichier dans la ressource
Mammiferes_v2b_01530
Auteur(s)
  • Roger S. Holmes 1
  • Roland A. H. van Oorschot 1
  • John L. VandeBerg 1
Affiliation(s)
  • 1) Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78228-0147, San Antonio, Texas
Résumé

Polyacrylamide gel isoelectric focusing (PAGE-IEF), cellulose acetate electrophoresis, and histochemical techniques were used to examine the tissue and subcellular distribution, genetics and biochemical properties of aldehyde dehydrogenase (ALDH) isozymes in a didelphid marsupial, the gray short-tail opossum (Monodelphis domestica). At least 14 zones of activity were resolved by PAGE-IEF and divided into five isozyme groups and three ALDH classes, based upon comparisons with properties previously reported for human, baboon, rat, and mouse ALDHs. Opossum liver ALDHs were distributed among cytosol (ALDHs 1 and 5) and large granular (mitochondrial) fractions (ALDHs 2 and 5). Similarly, kidney ALDHs were distributed between the cytosol (ALDH5) and the mitochondrial fractions (ALDHs 2, 4, and 5), whereas a major isozyme (ALDH3), found in high activity in cornea, esophagus, ear pinna, tail, and stomach extracts, was localized predominantly in the cytosol fraction. Phenotypic variants of the latter enzyme were shown to be inherited in a normal Mendelian fashion, with two alleles at a single locus (ALDH3) showing codominant expression. The data provided evidence for genetic identity of corneal, ear pinna, tail, and stomach ALDH3 and supported biochemical evidence from other mammalian species that this enzyme has a dimeric subunit structure.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - evolutionary biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Molecular Biology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Ecology, Evolution, Behavior and Systematics
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Biochemistry
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
D10795A26E8E65ED734AFF332D6605407AF26BF1
Revue

Biochemical Genetics

Année de publication
1991
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.208
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-2P1PB74S-P
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