Nom du corpus

Corpus Systématique Animale

Titre du document

Dirofilaria immitis : Detection of parasite-specific antigen by monoclonal antibodies in glomerulonephritis in infected dogs

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Vers_v2b_00365
Auteur(s)
  • K. Nakagaki 1
  • S. Nogami 2
  • Y. Hayashi 3
  • B. Hammerberg 1
  • H. Tanaka 3
  • I. Ohishi 4
Affiliation(s)
  • 1) Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, 27606, Raleigh, NC, USA
  • 2) Laboratory of Veterinary Zoology, College of Veterinary Medicine, Nihon University, 252, Fujisawa, Kanagawa, Japan
  • 3) Laboratory of Veterinary Embryology and Anatomy, School of Veterinary Medicine, the University of Tokyo, Yayoi, 116, Bunkyou-Ku, Tokyo, Japan
  • 4) Laboratory of Veterinary Internal Medicine, School of Veterinary Medicine, Tokyo University of Agriculture and Technology, 183, Fuchu, Tokyo, Japan
Résumé

For the identification of circulating parasite antigens associated with immune-complex glomerulone-phritis in dogs infected withDirofilaria immitis, monoclonal antibodies (mAbs) were generated against adult worms. A total of 11 mAbs were selected for cloning because of their high productivity and their lack of cross-reactivity withToxocara canis in indirect immunofluorescence tests. The ability of mAbs to detect circulating antigens was examined using an antigen-capture enzyme-linked immunosorbent assay. Of the 11 mAbs, only NAK-1, an IgG2a mAb, was capable of detecting circulating antigens in 75% of infected dogs. However, this mAb was highly species-specific in its detection of circulating antigens, since sera from dogs infected with other nematode parasites were negative. Furthermore, the mAb detected antigens at the same glomerular sites in which IgG and/or C3 were deposited. The antigen deposits were observed along capillaries and/or in mesangial cells. The epitope recognized by this mAb is probably a carbohydrate, as it remained stable for 1 h at 100° C and was sensitive to periodate treatment. Two bands of 62 and 26 kDa, respectively, were detected on Western blots by the mAb when sera from dogs infected withD. immitis were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transblotted.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - mycology & parasitology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Infectious Diseases
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Insect Science
  • 1 - Health Sciences ; 2 - Veterinary ; 3 - General Veterinary
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
  • 1 - Life Sciences ; 2 - Immunology and Microbiology ; 3 - Parasitology
Catégories WoS
  • 1 - science ; 2 - parasitology
Identifiant ISTEX
44AB0A0EDB1041B1CF7F3A332018AF7E751EE1EF
Revue

Parasitology Research

Année de publication
1993
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.129
Sous-corpus
  • Vers
Type de publication
Journal
ark:/67375/1BB-1GVGLNPL-8
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