Nom du corpus

Corpus Systématique Animale

Titre du document

Short-term culture of spleen lymphocytes for chromosome analysis of small rodents

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_02528
Auteur(s)
  • M. Jotterand Bellomo 1
  • D. Mühlematter 1
  • M. Nabholz 2
Affiliation(s)
  • 1) Division de Génétique Médicale, Centre Hospitalier Universitaire Vaudois (CHUV), 1011, Lausanne, Switzerland
  • 2) Institut Suisse de Recherches Expérimentales sur le Cancer (ISREC), 1066, Epalinges, Switzerland
Résumé

In order to improve the methods for the cytogenetic analysis of small rodents with regard to metaphase number, quality of chromosome resolution and duration of cultivation, a short-term culture technique from spleen cells, based on the capacity of Concanavalin A supernatant to stimulate the proliferation of normal T lymphocytes, was developed. Protocols of culture and chromosome preparation are described in detail. This technique not only makes it possible to obtain a high number of cells in mitosis from the spleen of very small rodents in a short time, but also insures metaphases of good quality, due to optimal control of cell cycle and processing thanks to cultivation in small aliquots. Although the method applies mainly to rodents because of species specificity of interleukin 2, it can probably be easily adapted to species from other mammalian orders.

Catégories Science-Metrix
  • 1 - natural sciences
  • 2 - biology
  • 3 - evolutionary biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
  • 4 - psychologie. psychophysiologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Insect Science
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Plant Science
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - Animal Science and Zoology
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
Identifiant ISTEX
0D7BC438A036F2DC6AC935FE9D49DB8C17553468
Revue

Genetica

Année de publication
1990
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
6.964
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-17L3NC8H-5
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