Nom du corpus

Corpus Systématique Animale

Titre du document

Immunocytochemical localization of platelets in baboon hepatic sinusoids using monoclonal mouse anti-human platelet glycoprotein IIIa following induction of thrombocytopenia

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Reptiles_v2b_00893
Auteur(s)
  • J. D. Frank 1
  • R. J. Gould 2
  • L. W. Schaffer 3
  • J. T. Davidson 4
  • R. E. Gibson 4
  • D. H. Patrick 1
  • S. L. Vonderfecht 1
  • M. E. Cartwright 1
Affiliation(s)
  • 1) Department of Safety Assessment, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA, USA
  • 2) Department of Biological Chemistry, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA, USA
  • 3) Department of Pharmacology, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA, USA
  • 4) Department of Radiopharmacology, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA, USA
Résumé

A commercially available mouse monoclonal antibody to human platelet glycoprotein IIIa was used to demonstrate sequestration of platelets in hepatic biopsies obtained from baboons following intravenous infusion of echistatin, a novel fibrinogen receptor antagonist derived from the venom of the snake Echis carinatus. Biopsies of liver and spleen were taken prior to administration of echistatin. The hepatic biopsies were either snap-frozen in Freon-22/liquid nitrogen or fixed in 10% neutral buffered formalin. Biopsies of spleen were snapfrozen. During infusion of echistatin (2.3 µg/kg/min), circulating platelet counts decreased from 331000/mm3 to 167000/mm3. Selective sequestration within the liver was confirmed using whole body gamma camera imaging to demonstrate 111Indium-oxine labeled platelet accumulation within the liver during the thrombocytopenic episode. Hepatic biopsies were again taken and either snap-frozen in Freon-22/liquid nitrogen or fixed in 10% neutral buffered formalin. Biopsies of spleen and inguinal lymph node were also snap-frozen. Platelet rich plasma smears, included as positive controls, dewaxed paraffin sections, and cryosections of liver, spleen, and lymph node were stained with monoclonal mouse anti-human platelet glycoprotein IIIa using an avidin biotinylated peroxidase complex (ABC) technique. Prior to infusion of echistatin, platelet staining within the liver was minimal. After echistatin infusion, hepatic cryosections showed prominent platelet staining within hepatic sinusoids. No localization was shown in lymph node, however, the spleen showed prominent platelet staining both before and after echistatin infusion. Platelet rich plasma smears were intensely positive. No prominent platelet staining was observed in formalin-fixed, paraffin-embedded material. Thus, this immunocytochemical technique may help localize platelets in cryosections of tissues from baboons and other primate species.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - biochemistry & molecular biology
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences medicales
  • 4 - radiotherapie. traitement instrumental. physiotherapie. reeducation. readaptation, orthophonie, crenotherapie. traitement dietetique et traitements divers (generalites)
Catégories Scopus
  • 1 - Life Sciences ; 2 - Agricultural and Biological Sciences ; 3 - General Agricultural and Biological Sciences
  • 1 - Health Sciences ; 2 - Medicine ; 3 - General Medicine
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Anatomy
Identifiant ISTEX
B9AC4FCE11636A2693CB4A72E46F5953D9633FC6
Revue

Histochemistry

Année de publication
1992
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
8.882
Sous-corpus
  • Reptiles
Type de publication
Journal
ark:/67375/1BB-0F1641BM-C
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