Nom du corpus

Corpus Systématique Animale

Titre du document

Phosphoglycerate kinase pseudogenes in the tammar wallaby and other macropodid marsupials

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_02233
Auteur(s)
  • D. W. Cooper 1
  • E. A. Holland 1
  • K. Rudman 1
  • J. A. Donald 1
  • R. Zehavi-Feferman 1
  • L. M. McKenzie 1
  • A. H. Sinclair 2
  • J. A. Spencer 2
  • J. A. M. Graves 2
  • W. E. Poole 3
Affiliation(s)
  • 1) School of Biological Sciences, Macquarie University, 2109, New South Wales, Australia
  • 2) School of Biological Sciences, La Trobe University, 3083, Bundoora, Victoria, Australia
  • 3) CSIRO Division of Wildlife and Ecology, 2602, Lyneham, Australian Capital Territory, Australia
Résumé

Phosphoglycerate kinase (EC 2.7.2.3; PGK) exists in two forms in marsupials. PGK1 is an X-linked house-keeping enzyme, and PGK2 is a mainly testis-specific enzyme under autosomal control. We have used PGK1 probes derived from two closely related species of macropodid marsupials (kangaroos and wallabies) to demonstrate the existence of a large family of pseudogenes in the tammar wallaby (Macropus eugenii). Over 30 fragments are detectable after Taq digestion. We estimate that there are 25–30 copies per genome. Most are autosomally inherited and are apparently not closely linked. Only two restriction fragments that appeared to be sex linked could be detected. Varying degrees of hybridization of fragments to the probes suggest different levels of homology, and hence different ages of origin. The existence of two PGK1 homologous restriction fragments from the X and a large number from the autosomes was also demonstrated by somatic cell hybridization for two other macropodid species, the wallaroo (M. robustus) and the red kangaroo (M. rufus). These results are compared with those from human and mouse, and it is suggested that the propensity of PGK1 to form pseudogenes is an ancient (?130 MYR BP) characteristic of mammals. The high level of polymorphism detected in the tammar makes these PGK1 probes potentially useful for measuring genetic variability in this species and other macropodids.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - genetics & heredity
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
  • 1 - science ; 2 - biotechnology & applied microbiology
  • 1 - science ; 2 - biochemistry & molecular biology
Identifiant ISTEX
928FAFCB46D7FB5F13FE0E7B15A2625969348B89
Revue

Mammalian Genome

Année de publication
1994
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.265
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-0BK703WQ-X
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