Nom du corpus

Corpus Systématique Animale

Titre du document

Expression and chromosomal mapping of the gene encoding the human histone H1.1

Lien vers le document
Éditeur
Springer (journals)
Langue(s) du document
Anglais
Type de document
Research-article
Nom du fichier dans la ressource
Mammiferes_v2b_00828
Auteur(s)
  • P. Burfeind 1
  • S. Hoyer-Fender 1
  • D. Doenecke 2
  • C. Hochhuth 2
  • W. Engel 1
Affiliation(s)
  • 1) Institut für Humangenetik der Universität, Goßlerstrasse 12d, D-37073, Göttingen, Germany
  • 2) Institut für Biochemie und Molekulare Zellbiologie der Universität, Abteilung Molekularbiologie, Humboldtallee 23, D-37073, Göttingen, Germany
Résumé

The expression of a human histone H1 isoform (H1.1) was studied in several human tissues. Northern blot analysis has revealed that this gene is expressed in testis and thymus, but not in other human tissues. In this report, we demonstrate that the expression of the histone H1.1 gene in human testis is restricted to early round spermatids that belong to the fraction of postmeiotic sperm cells. Transcripts hybridizing with the human H1.1 gene could not be detected in testis of mouse, rat, bull or boar. Southern blot analysis with human genomic DNA, DNA from different Old World monkeys (chimpanzee, orangutan, gorilla and rhesus monkey) and DNA from several mammalian species has revealed that the histone H1.1 gene is highly conserved in higher primates, whereas no cross-hybridization can be detected with DNA from other mammalian species such as mouse, rat, hamster or bull. In a previous report, the human histone H1.1 gene and other H1 genes (H1.2–H1.5, H1t) were assigned to chromosome 6 by polymerase chain reaction analysis using human-rodent cell hybrid DNA; fluorescence in situ hybridization indicated that these genes form part of a major gene cluster on the short arm of chromosome 6. We have confirmed the localization of histone H1.1 to chromosome 6 and have regionally assigned the locus to 6p21.3 by radioactive in situ hybridization.

Catégories Science-Metrix
  • 1 - health sciences
  • 2 - biomedical research
  • 3 - genetics & heredity
Catégories INIST
  • 1 - sciences appliquees, technologies et medecines
  • 2 - sciences biologiques et medicales
  • 3 - sciences biologiques fondamentales et appliquees. psychologie
Catégories Scopus
  • 1 - Health Sciences ; 2 - Medicine ; 3 - Genetics(clinical)
  • 1 - Life Sciences ; 2 - Biochemistry, Genetics and Molecular Biology ; 3 - Genetics
Catégories WoS
  • 1 - science ; 2 - genetics & heredity
Identifiant ISTEX
CC74A376759FA3504B8F9D0FC48EF053A59635E1
Revue

Human Genetics

Année de publication
1994
Présence de XML structuré
Non
Version PDF
1.3
Score qualité du texte
9.011
Sous-corpus
  • Mammiferes
Type de publication
Journal
ark:/67375/1BB-09R9PBGF-N
Powered by Lodex 9.3.8